5X2T
Direct Observation of Conformational Population Shifts in Hemoglobin: Crystal Structure of Half-Liganded Hemoglobin after Adding 4 mM bezafibrate pH 7.2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-23 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 228.494, 55.024, 138.504 |
Unit cell angles | 90.00, 103.38, 90.00 |
Refinement procedure
Resolution | 48.770 - 2.640 |
R-factor | 0.234 |
Rwork | 0.232 |
R-free | 0.27700 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.640 |
Number of reflections | 48972 |
<I/σ(I)> | 11.6 |
Completeness [%] | 98.1 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.7 | 293 | 17%(w/v) PEG 3350, pH 7.7 |