5X2L

Crystal Structure of Human Serine Racemase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-02
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	80.120, 112.590, 88.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.062 - 1.806
R-factor	0.2221
Rwork	0.220
R-free	0.25810
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.062
High resolution limit [Å]	1.806
Number of reflections	73388
<I/σ(I)>	9.1
Completeness [%]	100.0
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% PEG 8000, 5mM MgCl2, 10% Ethylene Glycol, 0.1M bis-Tris pH6.0