5X2I
Polygalacturonate Lyase by Fusing with a Self-assembling Amphipathic Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2015-12-26 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 41.094, 51.212, 51.296 |
Unit cell angles | 67.51, 74.93, 78.75 |
Refinement procedure
Resolution | 28.427 - 2.050 |
R-factor | 0.17709 |
Rwork | 0.176 |
R-free | 0.19952 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1bn8 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.703 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.427 |
High resolution limit [Å] | 1.860 |
Number of reflections | 18202 |
<I/σ(I)> | 12.1 |
Completeness [%] | 91.8 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 293 | 0.2 M sodium bromide, 20 mM Glycine-NaOH, 20 % polyethylene glycol 3350, 7 mM calcium chloride, 20 % glycerol |