5X27
Crystal structure of EGFR 696-1022 L858R in complex with SKLB(5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97923 |
Spacegroup name | I 2 3 |
Unit cell lengths | 145.384, 145.384, 145.384 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.974 - 2.952 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.23840 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.339 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.950 |
Number of reflections | 10571 |
<I/σ(I)> | 16.6 |
Completeness [%] | 96.4 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.1M Hepes pH 7.8, 40% PEG 400, 0.15M NaCl, 5mM tris(2-carboxyethyl)-phosphine (TCEP) |