5X1H
Structure of Legionella pneumophila DotN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-24 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.2828 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 155.357, 155.357, 527.711 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.934 - 3.005 |
R-factor | 0.2572 |
Rwork | 0.256 |
R-free | 0.29730 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.408 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
Rmerge | 0.104 | 0.071 | 0.317 |
Rmeas | 0.107 | 0.072 | 0.406 |
Rpim | 0.023 | 0.013 | 0.249 |
Total number of observations | 777894 | ||
Number of reflections | 109241 | ||
<I/σ(I)> | 14.5 | ||
Completeness [%] | 85.4 | 98.2 | 60.9 |
Redundancy | 12 | 27.1 | 1.8 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 600mM sodium citrate and 100mM sodium cacodylate (pH 6.5) |