5X1C
Crystal Structure of Human CRMP-2 without C-terminal Tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-13 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 126.687, 126.687, 148.219 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.263 - 2.101 |
R-factor | 0.1793 |
Rwork | 0.177 |
R-free | 0.20160 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.199 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 70654 |
<I/σ(I)> | 3.5 |
Completeness [%] | 100.0 |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium sulfate, Tris pH 7.0 |