5X0J
Free serine kinase (E30Q mutant) in complex with phosphoserine and AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2016-02-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.824, 43.400, 157.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.430 |
| R-factor | 0.18134 |
| Rwork | 0.180 |
| R-free | 0.19827 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5x0e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.369 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.430 |
| Number of reflections | 45510 |
| <I/σ(I)> | 21.7 |
| Completeness [%] | 97.5 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 293 | PEG 3350, serine |
