5X0E
Free serine kinase (E30A mutant) in complex with phosphoserine and AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-13 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.667, 43.224, 157.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.18298 |
| Rwork | 0.181 |
| R-free | 0.22188 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vk1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.396 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 16587 |
| <I/σ(I)> | 24.3 |
| Completeness [%] | 95.9 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 293 | PEG 3350, serine |
