5X00
Nucleoside Diphosphate Kinase from Vibrio cholerae is a Thermolabile Type II tetramer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-12-03 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.370, 71.210, 89.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.637 - 3.060 |
R-factor | 0.2298 |
Rwork | 0.228 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4s0m |
RMSD bond length | 0.002 |
RMSD bond angle | 0.464 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless (1.10.26) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.640 | 3.310 |
High resolution limit [Å] | 3.060 | 3.060 |
Rmerge | 0.180 | 0.710 |
Rpim | 0.090 | 0.490 |
Number of reflections | 5538 | 1162 |
<I/σ(I)> | 2.1 | 2.1 |
Completeness [%] | 91.4 | 94.9 |
Redundancy | 4.6 | 4.5 |
CC(1/2) | 0.900 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 285 | 0.2 M Sodium acetate, 0.1 M Tris-HCl pH 8.5, 30% PEG 4000 |