5WZK

Structure of APUM23-deletion-of-insert-region-GGAAUUGACGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 65
Unit cell lengths	189.060, 189.060, 45.456
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.288 - 2.800
R-factor	0.1827
Rwork	0.180
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wzg
RMSD bond length	0.008
RMSD bond angle	0.973
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.850
High resolution limit [Å]	2.800	7.580	2.800
Rmerge	0.144	0.037	0.838
Rmeas	0.153	0.039	0.888
Rpim	0.051	0.013	0.292
Number of reflections	23439
<I/σ(I)>	3.1
Completeness [%]	100.0	100	100
Redundancy	9	8.2	9.2
CC(1/2)		0.999	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M Lithium sulfate monohydrate, 0.1M Bis-Tris (pH 6.5), 25% w/v Polyethylene glycol 3350