5WZH
Structure of APUM23-GGAAUUGACGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 65 |
| Unit cell lengths | 188.409, 188.409, 45.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.884 - 2.509 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wzg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.911 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.115 | 0.042 | 0.763 |
| Rmeas | 0.121 | 0.044 | 0.798 |
| Rpim | 0.034 | 0.012 | 0.227 |
| Total number of observations | 392238 | ||
| Number of reflections | 31090 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 96.5 | 94 | 97.3 |
| Redundancy | 12.6 | 12.5 | 12.8 |
| CC(1/2) | 0.998 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.15 M DL-Malic acid (pH 7.0), 20% w/v Polyethylene glycol 3350 |
