5WZG

Structure of APUM23-GAAUUGACGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97776
Spacegroup name	P 65
Unit cell lengths	187.067, 187.067, 45.269
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.166 - 2.550
R-factor	0.1829
Rwork	0.181
R-free	0.21690
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.941
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.590
High resolution limit [Å]	2.550	6.910	2.550
Rmerge	0.120	0.038	0.620
Rmeas	0.126	0.039	0.655
Rpim	0.038	0.012	0.208
Total number of observations	313477
Number of reflections	30133
<I/σ(I)>	3.3
Completeness [%]	99.8	99.1	99.9
Redundancy	10.4	10.8	9.5
CC(1/2)		0.999	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.15 M DL-Malic acid (pH 7.0), 20% w/v Polyethylene glycol 3350