5WYB
Structure of Pseudomonas aeruginosa DspI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 125.262, 125.262, 72.651 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.758 - 2.250 |
| R-factor | 0.2337 |
| Rwork | 0.230 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fzw |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.296 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1-2155_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.758 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 16394 | 1330 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 20.5 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 10% isopropanol, 5% MPD, 0.1M CH3COONa pH 5.0 0.5M (NH4)2SO4, 0.1M CH3COONa pH 4.5 |
