5WYB
Structure of Pseudomonas aeruginosa DspI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2015-12-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 125.262, 125.262, 72.651 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.758 - 2.250 |
R-factor | 0.2337 |
Rwork | 0.230 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fzw |
RMSD bond length | 0.015 |
RMSD bond angle | 1.296 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1-2155_1692: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.758 | 2.310 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 16394 | 1330 |
<I/σ(I)> | 19 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 20.5 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 10% isopropanol, 5% MPD, 0.1M CH3COONa pH 5.0 0.5M (NH4)2SO4, 0.1M CH3COONa pH 4.5 |