5WY3

Crystal structure of Chaetomium thermophilum Utp10 middle domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 65 2 2
Unit cell lengths	91.052, 91.052, 554.758
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.947 - 3.200
R-factor	0.2259
Rwork	0.222
R-free	0.26570
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.397
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11rc1_2513: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.947	19.947	3.390
High resolution limit [Å]	3.200	8.740	3.200
Rmerge	0.101	0.043	1.189
Rmeas	0.103	0.044	1.220
Number of reflections	23700	1916	6682
<I/σ(I)>	21.1	72.87	2.66
Completeness [%]	99.2	91.5	99.1
Redundancy	20.753
CC(1/2)	1.000	0.999	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Hepes-Na, 2.0 M ammonium sulfate