5WY1
Crystal structure of mouse DNA methyltransferase 1 (T1505A mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-17 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.900 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 134.998, 97.804, 130.319 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.270 |
R-factor | 0.2061 |
Rwork | 0.203 |
R-free | 0.26270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3av4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.415 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.320 |
High resolution limit [Å] | 3.260 | 8.830 | 3.260 |
Rmerge | 0.190 | 0.049 | 0.748 |
Rmeas | 0.219 | ||
Rpim | 0.106 | ||
Number of reflections | 26720 | ||
<I/σ(I)> | 5.4 | ||
Completeness [%] | 97.5 | 92.7 | 96.4 |
Redundancy | 4.4 | 4.1 | 4.4 |
CC(1/2) | 0.992 | 0.355 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | PEG 3350, NaCl, TCEP, Na-citrate |