5WXX
Crystal structure of Microcystis aeruginosa PCC 7806 aspartate racemase in complex with citrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97930 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 128.751, 128.751, 46.182 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.667 - 2.350 |
R-factor | 0.18732 |
Rwork | 0.187 |
R-free | 0.20217 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.165 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.500 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 18567 | |
<I/σ(I)> | 32.4 | |
Completeness [%] | 99.9 | |
Redundancy | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 25% (w/v) polyethylene glycol 3350, 0.1 M sodium citrate, pH 6.5, 0.2 magnesium chloride |