5WXL

Crystal structure of the Rrs1 and Rpf2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00903
Spacegroup name	P 32 2 1
Unit cell lengths	71.791, 71.791, 268.905
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.805 - 1.900
R-factor	0.1808
Rwork	0.179
R-free	0.21050
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.866
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.11rc1_2513: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.930
High resolution limit [Å]	1.900	5.130	1.900
Rmerge	0.084	0.042	0.515
Total number of observations	387229
Number of reflections	64639
<I/σ(I)>	10.1
Completeness [%]	99.9	98.9	99.7
Redundancy	6	5.6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M Trimethylamine N-oxide, 0.1 M Tris-HCl, 20% PEG2000