5WXI

EarP bound with dTDP-rhamnose (soaked)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	90
Detector technology	CCD
Collection date	2016-02-23
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	97.578, 195.388, 46.366
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.847 - 2.000
R-factor	0.173
Rwork	0.172
R-free	0.21320
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	apo EarP
RMSD bond length	0.006
RMSD bond angle	0.850
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.850	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	61063
<I/σ(I)>	10.7	1.9
Completeness [%]	100.0	99.7
Redundancy	7.3	7.2
CC(1/2)		0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Hepes-NaOH buffer (pH 6.8-7.5), 20% PEG3350, 0.2M ammonium sulfate