5WXG

Structure of TAF PHD finger domain binds to H3(1-15)K4ac

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	50.840, 52.868, 54.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.344 - 1.703
R-factor	0.1695
Rwork	0.167
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.928
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10_2155: ???)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.101	0.042	0.738
Number of reflections	8164
<I/σ(I)>	9.3
Completeness [%]	98.7	93	100
Redundancy	4.5	4.3	4.5
CC(1/2)		0.998	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.03M magnesium chloride, 0.03M calcium chloride, 0.1M MES, 0.1M imidazole, PH6.5, 15% PEGMME 550, 15% PEG 20K