5WWZ

Crystal structure of the KH2 domain of human RNA-binding E3 ubiquitin-protein ligase MEX-3C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 42 21 2
Unit cell lengths	83.321, 83.321, 78.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.262 - 2.500
R-factor	0.2284
Rwork	0.226
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5www
RMSD bond length	0.002
RMSD bond angle	0.430
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.082	0.039	0.563
Rmeas	0.089	0.042	0.609
Rpim	0.033	0.016	0.230
Total number of observations	70047
Number of reflections	10139
<I/σ(I)>	4.4
Completeness [%]	100.0	99.9	100
Redundancy	6.9	6.4	6.9
CC(1/2)		0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.6M Ammonium sulfate