5WWU
Crystal Structure of HLA-A*2402 in complex with 2009 pandemic influenza A(H1N1) virus and avian influenza A(H5N1) virus-derived peptide H1-25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-10 |
Detector | SDMS |
Wavelength(s) | 0.97774 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.752, 79.099, 88.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.506 - 2.794 |
R-factor | 0.2275 |
Rwork | 0.225 |
R-free | 0.28420 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.703 |
Data reduction software | CNS |
Data scaling software | CNS |
Phasing software | CNS |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.794 |
Number of reflections | 138842 |
<I/σ(I)> | 35.2 |
Completeness [%] | 94.5 |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4000 |