5WW9
Crystal structure of R73E mutant of the second DNA-Binding protein under starvation from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-05-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | H 3 2 |
Unit cell lengths | 90.950, 90.950, 421.065 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.18394 |
Rwork | 0.182 |
R-free | 0.22376 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.154 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 41444 | |
<I/σ(I)> | 38.5 | 15.7 |
Completeness [%] | 89.9 | 82.2 |
Redundancy | 17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6 | 298 | 100 mM sodium cacodylate, 150 mM MgCl2, 20% PEG3350 |