5WVN
Crystal structure of MBS-BaeS fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2015-06-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 119.084, 119.084, 147.011 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.680 - 2.800 |
R-factor | 0.23 |
Rwork | 0.228 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.148 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.684 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 25337 | |
<I/σ(I)> | 39.3 | 3.2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 10.1 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Citric Acid: NaOH , Ammonium Sulfate |