5WV9

Crystal structure of a insect group III chitinase complex with (GlcNAc)6 (CAD1-(GlcNAc)6 ) from Ostrinia furnacalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	71.781, 71.781, 193.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.056 - 2.105
R-factor	0.1697
Rwork	0.168
R-free	0.19970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wup
RMSD bond length	0.010
RMSD bond angle	1.368
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	30323
<I/σ(I)>	9
Completeness [%]	99.9	100
Redundancy	14.1	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	200mM ammonium sulfate, 100mM bis-Tris, pH 6.5, 20% PEG3350