5WV8
Crystal structure of a mutant insect group III chitinase (CAD1-E217L) from Ostrinia furnacalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.100, 72.100, 192.997 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.099 - 2.042 |
R-factor | 0.1661 |
Rwork | 0.165 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wup |
RMSD bond length | 0.007 |
RMSD bond angle | 0.987 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 35271 | |
<I/σ(I)> | 10.6 | |
Completeness [%] | 94.4 | 85 |
Redundancy | 14.1 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 200mM ammonium sulfate, 100mM Bis-Tris pH 6.6, 21% PEG 3350 |