5WUW
Serratia marcescens short-chain dehydrogenase/reductase F98L/F202L mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2016-10-21 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 84.012, 84.012, 115.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 1.900 |
R-factor | 0.17358 |
Rwork | 0.172 |
R-free | 0.21174 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zgw |
RMSD bond length | 0.020 |
RMSD bond angle | 1.990 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 33651 |
<I/σ(I)> | 10.5 |
Completeness [%] | 92.3 |
Redundancy | 26.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50 mM HEPES buffer (pH 7.0), 40% (v/v) tacsimate (pH 7.0), 2 mM spermine, and 2 mM hexamine cobalt (III) chloride |