5WUM
Crystal structure of mouse importin-alpha1 bound to S385-phosphorylated NLS of EBNA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2016-05-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.910, 90.300, 99.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.065 - 2.000 |
R-factor | 0.1872 |
Rwork | 0.186 |
R-free | 0.21150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ukw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.630 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.8) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.970 | 41.970 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.059 | 0.015 | 0.863 |
Rmeas | 0.065 | ||
Rpim | 0.026 | ||
Total number of observations | 288684 | ||
Number of reflections | 48181 | ||
<I/σ(I)> | 19 | ||
Completeness [%] | 99.9 | 99.1 | 99.3 |
Redundancy | 6 | 5.2 | 4.8 |
CC(1/2) | 0.999 | 1.000 | 0.653 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES, sodium citrate, DTT |