5WUF
Structural basis for conductance through TRIC cation channels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97876 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 91.312, 91.312, 252.799 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.736 - 2.401 |
| R-factor | 0.2416 |
| Rwork | 0.240 |
| R-free | 0.27350 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.424 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | PHENIX (dev_2481) |
| Refinement software | PHENIX (dev_2481) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.660 | 45.660 | 2.490 |
| High resolution limit [Å] | 2.400 | 8.980 | 2.400 |
| Rmerge | 0.197 | 0.077 | 0.454 |
| Number of reflections | 30649 | ||
| <I/σ(I)> | 20.8 | ||
| Completeness [%] | 99.8 | 99.4 | 98.1 |
| Redundancy | 73.6 | 60.2 | 40.1 |
| CC(1/2) | 1.000 | 1.000 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | PEG400 38%, NaCl 100mM, CdCl2 100mM, Tris-HCl, pH 8.5, 100mM |
