5WUE

Structural basis for conductance through TRIC cation channels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91532
Spacegroup name	H 3 2
Unit cell lengths	115.872, 115.872, 134.753
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.509 - 2.400
R-factor	0.2261
Rwork	0.225
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wuc
RMSD bond length	0.008
RMSD bond angle	0.974
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	PHENIX (dev_2481)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.060	47.060	2.600
High resolution limit [Å]	2.400	9.020	2.500
Rmerge	0.020	0.067	0.243
Number of reflections	23120
<I/σ(I)>	15.6
Completeness [%]	89.2	99.3	94.6
Redundancy	25.9	23.4	20.3
CC(1/2)	0.998	0.998	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	291	PEG 200 40%, Li2SO4, 100mM, HEPES pH 7.0 100mM