5WUC

Structural basis for conductance through TRIC cation channels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97876
Spacegroup name	P 63
Unit cell lengths	64.253, 64.253, 80.811
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.695 - 1.600
R-factor	0.22
Rwork	0.219
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	PHENIX (dev_2481)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.030	46.030	1.840
High resolution limit [Å]	1.600	9.000	1.800
Rmerge	0.025	0.033	0.049
Number of reflections	48871
<I/σ(I)>	14.5
Completeness [%]	100.0	99.4	99.4
Redundancy	20.3	21	20.4
CC(1/2)	0.999	1.000	0.318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	291	PEG 200 40%, NaCl 100mM, MgCl2 100mM, MES pH 6.0 100mM