5WU7
Crystal structure of GH57-type branching enzyme from hyperthermophilic archaeon Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.857, 42.326, 122.227 |
| Unit cell angles | 90.00, 90.96, 90.00 |
Refinement procedure
| Resolution | 33.521 - 2.310 |
| R-factor | 0.2028 |
| Rwork | 0.200 |
| R-free | 0.25970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n8t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.554 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11_2567)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.521 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.383 |
| Number of reflections | 44048 | |
| <I/σ(I)> | 10.2 | 2.08 |
| Completeness [%] | 95.0 | 72.1 |
| Redundancy | 5.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.3 | 290 | 20 mM citric acid, 10% (w/v) PEG 3350, 80 mM Bis-Tris propane pH 9.3, 2 mM tris (2-carboxyethyl) phosphine |
