5WU0
Crystal structure of C. perfringens iota-like enterotoxin CPILE-a with NADH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-10-29 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 70.583, 101.631, 125.297 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.499 - 2.251 |
R-factor | 0.1789 |
Rwork | 0.176 |
R-free | 0.24790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gtt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.123 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.251 |
Number of reflections | 21170 |
<I/σ(I)> | 41.3 |
Completeness [%] | 98.4 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 4000, Tris HCl, Magnesium chloride |