5WTT

Structure of the 093G9 Fab in complex with the epitope peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-08
Detector	RAYONIX MX-325
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	41.883, 214.437, 50.045
Unit cell angles	90.00, 92.81, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.22456
Rwork	0.222
R-free	0.27406
RMSD bond length	0.009
RMSD bond angle	1.135
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	23993
<I/σ(I)>	12.5
Completeness [%]	99.3
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	14% PEG 6000, 8% MPD, pH7.9, 5% ethyle acetate