5WTS
Green fluorescent protein linked MTide-02 inhibitor in complex with mdm2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 51.766, 98.422, 218.988 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.007 - 3.004 |
R-factor | 0.2061 |
Rwork | 0.204 |
R-free | 0.24420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.473 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.007 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.088 | 0.401 |
Number of reflections | 11255 | |
<I/σ(I)> | 14.25 | 2.21 |
Completeness [%] | 97.3 | 92.6 |
Redundancy | 4.7 | 4.2 |
CC(1/2) | 0.887 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 288 | 0.2M sodium chloride, 0.1M Bis-Tris, 25% PEG3350 |