5WTQ
Crystal structure of human proteasome-assembling chaperone PAC4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2016-07-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.90000 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 72.548, 152.779, 212.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.868 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.800 |
| Number of reflections | 46618 | |
| <I/σ(I)> | 42 | 3.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.0 M lithium sulfate, 0.1 M Tris-HCl (pH 8.5), and 10 mM nickel chloride |
