5WTN
Crystal Structure Analysis of primosome protein DnaB (resiues 1-300) from Geobacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-06 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.356, 118.980, 157.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.213 - 2.800 |
R-factor | 0.2297 |
Rwork | 0.228 |
R-free | 0.26250 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.353 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.8_1069) |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.083 |
Number of reflections | 52235 |
<I/σ(I)> | 53.4 |
Completeness [%] | 99.6 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 0.1M Tris (pH 8.5), 1.0M Ammonium sulfate |