5WTB
Complex Structure of Staphylococcus aureus SdrE with human complement factor H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.500, 117.500, 154.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.790 - 3.300 |
| R-factor | 0.22483 |
| Rwork | 0.222 |
| R-free | 0.28097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r17 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.371 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.790 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 31291 | |
| <I/σ(I)> | 7 | 1.9 |
| Completeness [%] | 96.1 | 97.9 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M HEPES sodium pH 7.5, 0.2M Magnesium chloride, 30% PEG 400 (v/v) |
