5WSY
The complex structure of SAV606 with N-carboxymethyl-3-aminobutyrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.495, 118.313, 71.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.570 - 2.000 |
R-factor | 0.17998 |
Rwork | 0.178 |
R-free | 0.21452 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pfc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.861 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.091 | 0.556 |
Number of reflections | 32063 | |
<I/σ(I)> | 21.4 | 2.4 |
Completeness [%] | 100.0 | 99.6 |
Redundancy | 6.5 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 1.0 M magnesium citrate, 0.1 M imidazole, 1% glycerol, 10 mM N-carboxymethyl-3-aminobutyrate |