5WSX
The crystal structure of SAV606
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.880, 50.100, 125.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.550 - 2.400 |
R-factor | 0.20728 |
Rwork | 0.205 |
R-free | 0.26113 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pfc |
RMSD bond length | 0.013 |
RMSD bond angle | 1.597 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.137 | 0.578 |
Number of reflections | 12859 | |
<I/σ(I)> | 10.8 | 2.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.5 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 1.0 M Magnesium citrate, 0.1 M imidazole, 1% glycerol |