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5WSV

Crystal structure of Myosin VIIa IQ5 in complex with Ca2+-CaM

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL17U
Synchrotron siteSSRF
BeamlineBL17U
Temperature [K]100
Detector technologyCCD
Collection date2014-04-12
DetectorADSC QUANTUM 315r
Wavelength(s)0.9791
Spacegroup nameP 1 21 1
Unit cell lengths38.683, 89.755, 45.724
Unit cell angles90.00, 106.03, 90.00
Refinement procedure
Resolution44.880 - 2.330
R-factor0.2298
Rwork0.228
R-free0.26230
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3g43
RMSD bond length0.012
RMSD bond angle1.246
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0155)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.390
High resolution limit [Å]2.3302.350
Rmerge0.0620.360
Number of reflections12459
<I/σ(I)>234.1
Completeness [%]97.598.9
Redundancy2.82.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION6.52890.2M ammonium sulfate, 0.1M Bis-Tris (pH 6.5), 25%(w/v) PEG 3,500

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