5WSV
Crystal structure of Myosin VIIa IQ5 in complex with Ca2+-CaM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.683, 89.755, 45.724 |
Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
Resolution | 44.880 - 2.330 |
R-factor | 0.2298 |
Rwork | 0.228 |
R-free | 0.26230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g43 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.246 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.330 | 2.350 |
Rmerge | 0.062 | 0.360 |
Number of reflections | 12459 | |
<I/σ(I)> | 23 | 4.1 |
Completeness [%] | 97.5 | 98.9 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 289 | 0.2M ammonium sulfate, 0.1M Bis-Tris (pH 6.5), 25%(w/v) PEG 3,500 |