5WSV
Crystal structure of Myosin VIIa IQ5 in complex with Ca2+-CaM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.683, 89.755, 45.724 |
| Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
| Resolution | 44.880 - 2.330 |
| R-factor | 0.2298 |
| Rwork | 0.228 |
| R-free | 0.26230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g43 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.246 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.350 |
| Rmerge | 0.062 | 0.360 |
| Number of reflections | 12459 | |
| <I/σ(I)> | 23 | 4.1 |
| Completeness [%] | 97.5 | 98.9 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 289 | 0.2M ammonium sulfate, 0.1M Bis-Tris (pH 6.5), 25%(w/v) PEG 3,500 |
