5WST
Crystal structure of Myo7a SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.810, 42.610, 59.680 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 32.554 - 2.100 |
| R-factor | 0.201 |
| Rwork | 0.195 |
| R-free | 0.23880 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.067 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.088 | 0.618 |
| Number of reflections | 11455 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 289 | 0.2M ammonium acetate, 0.1M sodium acetate (pH 4.6), 30%(w/v) PEG 4,000 |
