5WSL
Structural studies of keratinase from Meiothermus taiwanensis WR-220
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.210, 67.088, 92.857 |
| Unit cell angles | 90.00, 96.07, 90.00 |
Refinement procedure
| Resolution | 25.230 - 1.500 |
| R-factor | 0.1466 |
| Rwork | 0.145 |
| R-free | 0.17540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dzt |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.044 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 115261 | |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 97.3 | 95.6 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 292 | 0.2M lithium sulfate, 0.1M sodium acetate, 50% PEG 400 |
