5WS4
Crystal structure of tripartite-type ABC transporter MacB from Acinetobacter baumannii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-13 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.750 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 229.652, 229.652, 154.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.115 - 3.400 |
R-factor | 0.2285 |
Rwork | 0.227 |
R-free | 0.25710 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.819 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.086 | |
Number of reflections | 46498 | |
<I/σ(I)> | 27.5 | 1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 16.3 | 15.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 1.2M Sodium citric acid, 0.1M Sodium HEPES (pH7.2) |