5WRS
Crystal Structure of Fam20A in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 157.202, 157.202, 144.802 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.943 - 2.750 |
R-factor | 0.2042 |
Rwork | 0.203 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wrr |
RMSD bond length | 0.002 |
RMSD bond angle | 0.614 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.750 | 7.460 | 2.750 |
Rmerge | 0.168 | 0.059 | 1.470 |
Number of reflections | 55133 | ||
<I/σ(I)> | 9.9 | ||
Completeness [%] | 99.9 | 98.5 | 100 |
Redundancy | 10.7 | 10.3 | 10.6 |
CC(1/2) | 0.998 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | ammonium sulfate, HEPES, PEG 400 |