5WR1
Covalent bond formation of bifunctional ligand with hPPARg-LBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.000, 61.430, 118.500 |
| Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
| Resolution | 50.860 - 2.340 |
| R-factor | 0.23107 |
| Rwork | 0.229 |
| R-free | 0.25948 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.576 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.860 |
| High resolution limit [Å] | 2.340 |
| Number of reflections | 24512 |
| <I/σ(I)> | 17.1 |
| Completeness [%] | 88.4 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1M Tris-HCl PH 7.4, 0.8M sodium citrate |
