5WR0
Huisgen cycloaddition for PPARg-LBD labeling by soaking method
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.730, 61.210, 118.350 |
Unit cell angles | 90.00, 102.95, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.850 |
R-factor | 0.25229 |
Rwork | 0.250 |
R-free | 0.29975 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 115.340 |
High resolution limit [Å] | 2.850 |
Number of reflections | 12858 |
<I/σ(I)> | 12.3 |
Completeness [%] | 83.7 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1M Tris-HCl PH 7.4, 0.8M sodium citrate |