5WQF
Structure of fungal meroterpenoid isomerase Trt14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.610, 47.660, 121.440 |
| Unit cell angles | 90.00, 108.07, 90.00 |
Refinement procedure
| Resolution | 47.824 - 2.001 |
| R-factor | 0.2155 |
| Rwork | 0.214 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Selenomethionine labeled Trt14 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.787 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | |
| Number of reflections | 73543 | |
| <I/σ(I)> | 14.2 | 2.57 |
| Completeness [%] | 98.4 | 97.9 |
| Redundancy | 3.42 | 3.4 |
| CC(1/2) | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM Imidazole (pH 8.0), 10% PEG 8000, 100mM Ca(OAc)2, 2% 1-propanol |
