5WPA
Structure of human SFPQ/PSPC1 heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.954 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.229, 67.841, 92.204 |
Unit cell angles | 90.00, 95.06, 90.00 |
Refinement procedure
Resolution | 26.190 - 2.290 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sde |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.200 | 2.390 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.039 | 0.290 |
Rpim | 0.037 | 0.270 |
Number of reflections | 24463 | 2534 |
<I/σ(I)> | 12.8 | 2.5 |
Completeness [%] | 98.6 | 97 |
Redundancy | 3.7 | 3.7 |
CC(1/2) | 0.998 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG3350, 0.05 M MES, pH 5.5, 0.2 M sodium chloride, 3% aminohexanoic acid |