5WOS

Structural and functional insights into Canarypox Virus CNP058 regulation of apoptosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	73.786, 34.670, 71.726
Unit cell angles	90.00, 114.92, 90.00

Refinement procedure

Resolution	33.459 - 2.450
R-factor	0.214
Rwork	0.212
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tzp
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.460	2.550
High resolution limit [Å]	2.450	2.450
Rmerge	0.097	0.716
Rpim	0.084	0.710
Number of reflections	6021	703
<I/σ(I)>	6.1	1.1
Completeness [%]	97.2	98.4
Redundancy	2.7	2.7
CC(1/2)	0.994	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	0.2 M calcium chloride dihydrate, 0.1 M MES pH 6.0, 20% w/v PEG6000