5WOR
Corkscrew assembly of SOD1 residues 28-38 with familial mutation G37R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 59.615, 59.615, 98.927 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.770 - 2.770 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Four strands composed of residues VKVWGSI of PDBID 5DLI were used as initial model |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.800 | 2.900 |
High resolution limit [Å] | 2.770 | 2.800 |
Rmerge | 0.183 | 0.907 |
Number of reflections | 5444 | |
<I/σ(I)> | 8.9 | |
Completeness [%] | 98.9 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | reservoir contained 15% PEG 3350, 0.3M Sodium Malonate |